Agrobacterium tumefaciens-mediated transformation of Beauveria bassiana using an herbicide resistance gene as a selection marker Weiguo Fang, Yongjun Zhang, Xingyong Yang, Xuelian Zheng, Hui Duan, Yi Li, and Yan Pei Journal of Invertebrate Pathology 2004, 85:18–24
Cloning of Beauveria bassiana Chitinase Gene Bbchit1 and Its Application To Improve Fungal Strain Virulence Weiguo Fang, Bo Leng, Yuehua Xiao, Kai Jin, Jincheng Ma, Yanhua Fan,Jing Feng, Xingyong Yang, Yongjun Zhang, Yan Pei Applied and Environmental Microbiology 2005, 71(1):363–370
Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L) Zheng-Sheng Zhang, Yue-Hua Xiao, Ming Luo, Xian-Bi Li, Xiao-Ying Luo, Lei Hou, De-Mou Li, Yan Pei Euphytica 2005, 144: 91–99
The maize Knotted1 gene is an effective positive selectable marker gene for Agrobacterium-mediated tobacco transformation Keming Luo, Xuelian Zheng, Yongqin Chen, Yuehua Xiao, Degang Zhao, Richard McAvoy, Yan Pei, Yi Li Plant Cell Rep 2006,25:403-409
Leaf senescence is delayed in tobacco plants expressing the maize knotted1 gene under the control of a wound-inducible promoter Keming Luo, Wei Deng, Yuehua Xiao, Xuelian Zheng, Yi Li, Yan Pei Plant Cell Rep 2006, 25: 1246–1254
Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds Xingyong Yang, Jun Li, Xiaowen Wanga, Weiguo Fang, Michael J Bidochka, Rong She, Yuehua Xiao, Yan Pei Peptides 2006, 27(7):1726-1731
Isolation and characterization of a novel thermostable non-specific lipid transfer protein-like antimicrobial protein from motherwort (Leonurus japonicus Houtt) seeds Xingyong Yang, Jun Li, Xianbi Li, Rong She, Yan Pei Peptides 2006, 27: 3122-3128
Transformation of Metarizium anisopliae mediated by Agrobacterium tumefaciens Weiguo fang, Yan Pei and Michael Bidochka Can J Microbiol 2006, 52: 623-626
Direct amplification of intron-containing hairpin RNA construct from genomic DNA Yue-Hua Xiao, Meng-Hui Yin, Lei Hou, Yan Pei BioTechniques 2006, 41:548-552
Functional Expression of the Cotton Gibberellic Acid Oxidase Homologous Gene GhGA20ox1 in Tobacco XIAO Yue-Hua, YE Ying-Fu, FENG Yi, LI Xian-Bi LOU Ming, HOU Lei, LUO Xiao-Ying, LI De-Mou, PEI Yan Journal of Physiology and Molecular Biology 2006, 32(5): 563-569
Overexpression of an Arabidopsis magnesium transport gene, AtMGT1, in Nicotiana benthamiana confers Al tolerance Wei Deng, Keming Luo Demou Li, Xuelian Zheng, Xiaoyang Wei, William Smith, Chandra Thammina, Litang Lu, Yi Li, Yan Pei Journal of Experimental Botany 2006, 13: 4235-4243
In vitro regeneration and Agrobacterium-mediated genetic transformation of Euonymus alatus Yougqing Chen, Litang Lu, Wei Deng, Xingyu Yang, Richard MeAvoy, Degang Zhao, Yan Pei, Keming Luo,Hui Duan, William Smith, Chandra Thammina, Xuelian Zheng, Donna Ellis, Yi Li Plant Cell Rep 2006, 25:1043-1051
Increased Insect Virulence in Beauveria bassiana Strains Overexpressing an Engineered Chitinase Yanhua Fan, Weiguo Fang, Shujuan Guo, ,Xiaoqiong Pei, Yongjun Zhang, Yuehua Xiao, Demou Li, Kai Jin, Michael J Bidochka, Yan Pei Applied and Environmental Microbiology 2007,73(1): 295–302
‘GM-gene-deletor’: fused loxP-FRT recognition sequences dramatically improve the efficiency of FLP or CRE recombinase on transgene excision from pollen and seed of tobacco plants Keming Luo, Hui Duan, Degang Zhao, Xuelian Zheng, Wei Deng, Yongqin Chen, Neal Stewart Jr, Richard McAvoy, Xiangning Jiang, Yanhong Wu, Aigong He, Yan Pei, Yi Li Plant Biotechnology Journal 2007,5(2): 263-374
A regulator of a G protein signalling (RGS) gene, cag8, from the insect-pathogenic fungus Metarhizium anisopliae is involved in conidiation, virulence and hydrophobin synthesis Weiguo Fang, Yan Pei, Michael J Bidochka Microbiology 2007, 153: 1017–1025
Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis Yue-Hua Xiao, Meng-Hui Yin, Lei Hou, Ming Luo, Yan Pei Biotechnology Letters 2007 29: 925–930
Cotton flavonoid structural genes related to the pigmentation in brown fibers Yue-Hua Xiao, Zheng-Sheng Zhang, Meng-Hui Yin, Ming Luo, Xian-Bi Li, Lei Hou, Yan Pei Biochemical and Biophysical Research Communications 2007, 358: 73–78
The cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activity of adjacent tissue- and organ-specific gene promoters Xuelian Zheng, Wei Deng, Keming Luo, Hui Duan, Yongqin Chen, Richard McAvoy, Shuiqing Song, Yan Pei, Yi Li Plant Cell Rep 2007, 26: 1195–1203
Directed evolution for increased chitinase activity Yanhua Fan, Weiguo Fang, Yuehua Xiao, Xingyong Yang, Yongjun Zhang, Michael J Bidochka, Yan Pei Appl Microbiol Biotechnol 2007, 76: 135-139
GhDET2, a steroid 5a-reductase, plays an important role in cotton fiber cell initiation and elongation Ming Luo, Yuehua Xiao, Xianbi Li, Xiaofeng Lu, Wei Deng, Demou Li, Lei Hou, Mingyu Hu, Yi Li, Yan Pei The Plant Journal 2007, 51: 419–430
Sequential Amplification of Flanking Sequences by Y-shaped Adaptor Dependent Extension Using Multiple Templates XIAO Yue-Hua, PENG Yi, LUO Ming, LI De-Mou, HOU Lei, PEI Yan Journal of Plant Physiology and Molecular Biology 2007, 33(1): 85-90
Expression of a Beauveria bassiana chitinase (Bbchit1) in Escherichia coli and Pichia pastoris Yanhua Fan, Yongjun Zhang, Xingyong Yang, Xiaoqiong Pei, Shujuan Guo, Yan Pei Protein Expression and Purification 2007, 56: 93–99
Cloning and Characterization of a Balsam Pear Class I Chitinase gene (Mcchit1) and Its Ectopic Expression Enhances Fungal resistance in Transgenic Plants Yue-Hua Xiao, Xian-Bi Li, Xingyong Yang, Ming Luo, Lei Hou, Shao-Hui Guo, Xiao-Ying Luo, and Yan Pei Biosci Biotechnol Biochem 2007, 71(5): 1211 -1219
A cuticle-degrading protease (CDEP-1) of Beauveria bassiana enhances virulence Yong-Jun Zhang, Ming-Guang Fang, Yan-Hua Fan, Zhi-Bing Luo, Xing-Yong Yang, Di Wu and Yan Pei Biocontrol Science and Technology 2008, 18: 543-555
Implication of a regulator of Gprotein signaling (BbRGS1) in Conidiation and conidial thermotolerance of the insect pathogenic Fungus Beauveria bassiana Weiguo Fang, Lisa R Scully, Lei Zhang, Yan Pei and Michael J Bidochka FEMS Microbiol Lett 2008,279: 146–156
Functional characterization of a cotton late embryogenesis-abundant D113 gene promoter in transgenic tobacco Keming Luo, Guofang Zhang, Wei Deng, Fengtao Luo, Kun Qiu, Yan Pei Plant Cell Rep 2008, 27: 707-717
Characterization of a Highly Active Promoter, PBbgpd, in Beauveria bassiana Xing-gang Liao, Wei-guo Fang, Yong-jun Zhang, Yan-hua Fan, Xing-wei Wu, Qun Zhou, Yan Pei Current Microbiology 2008, 57: 121-126
An improved method for Beauveria bassiana transformation using phosphinothricin acetlytransferase and green fluorescent protein fusion gene as a selectable and visible marker Kai Jin, Yongjun Zhang, Zhibing Luo, Yuehua Xiao, Yanhua Fan, Di Wu, Yan Pei Biotechnol Lett 2008, 30: 1379–1383
SCFP, a novel fiber-specific promoter in cotton HOU Lei, LIU Hao, LI JiaBao, YANG Xia, XIAO YueHua, LUO Ming, SONG ShuiQing, YANG GuangWei and PEI Yan Chinese Science Bulletin 2008, 53(17): 2639-2645
Functional analysis of the Arabidopsis thaliana poly(A) binding protein PAB5 gene promoter in Nicotiana tabacum Keming Luo, Wei Deng, Shan Xu, Yan Pei Plant Cell Rep 2008 27: 1811-1819
Light stimulates conidiation of the entomopathogenic fungus Beauveria bassiana Yong-Jun Zhang, Zun-Hua Li, Zhi-Bing Luo, Jian-Qing Zhang, Yan-Hua Fan, Yan Pei Biocontrol Science and Technology 2009,19(1): 91-101
The size and ratio of homologous sequence to non-homologous sequence in gene disruption cassette influences the gene targeting efficiency in Beauveria bassiana Jin-Cheng Ma, Qun Zhou, Yong-Hong Zhou, Xing-Gang Liao, Yong-Jun Zhang, Dan Jin and Yan Pei Appl Microbiol Biotechnol 2009, 82: 891–898
Mitogen-Activated Protein Kinase hog1 in the Entomopathogenic Fungus Beauveria bassiana Regulates Environmental Stress Responses and Virulence to Insects Yong-Jun Zhang, Jianhua Zhao, Weiguo Fang, Jian-Qing Zhang, Zhi-Bing Luo, Mi Zhang, Yan-Hua Fan and Yan Pei Appl Envir Microbiol 2009, 75: 3787-3795
注:加的为通讯作者
面部清洁是为了更好的促进护肤品的吸收,所以洗面奶的选择也很重要。洗面奶上榜的都有哪些产品呢?清洁护肤产品是日常护肤必备,全球洗面奶品牌很多,看得人眼花缭乱。今天根据各品牌知名度和消费者好评和销量等多项数据盘点了口碑最好的女士洗面奶排行榜,希望可以帮助到你选到好用的洗面奶产品。
拉薇天然基础二合一洗面奶
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必买推荐!被很多护肤杂志称为国货之光的洗面奶!原料和配方都是和一线大牌一样的,这款是正宗的氨基酸成分比市面常见的洗面奶好很多,但是价格却是很亲民,很多护肤师都推荐过这个温净洗面奶。洗的干净洗完后皮肤很水润不紧绷,还能收缩毛孔,去黑头。最重要的是这个很温和,无刺激。无限回购款!
POLA黑BA洁面
膏状质地,会拉丝!泡沫丰富到挤出一丢丢就能搓出大量泡沫。用量真的是无敌省,关键是真的太舒服了!预算够的话强烈推荐!
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温和的泡沫洁面产品。蜡菊清香,干净舒服!不过泡沫类的洁面可能都不是很耐用,压压就没了。泡沫有点虚。
MUX纯风氨基酸洁面液
最喜欢的一款洁面产品!真的是深层清洁,洗出很多脏东西,黑头痘痘痘印暗沉用这个都有很好的效果!配方超级安心,敏感肌肤也可以放心用。卸妆也很干净,直接洗脸省去卸妆的麻烦。气味很好闻,便宜大瓶不心疼!近几年难得一见到好国货,不少粉丝都反馈用这个洗脸皮肤变好了,又白又光滑,男生女生都可以用,不用问!买它!
御泥坊玫瑰泡沫层清洁温和补水洗面奶
泡沫比较细腻清新不油腻,弱酸性接近皮肤的PH值,洗完后温和不刺激。大马革士玫瑰精华补水的效果还是不错的,一股淡淡的玫瑰花香味,性价比也还不错。
肌克祛痘水
最有效果的产品!医科大学实力研制,获得过多次祛痘金奖,效果和口碑极佳。如果有青春痘粉刺、闭口脓包、痘印痤疮、白头红肿都可以用这款解决,十几种天然植物精华保证祛痘效果,周围朋友七八年的痘痘痘印都是用它去掉的,一定要搭配肌克品牌的祛痘膏,这样效果好。特别喜欢这种无激素无化学刺激的产品,祛痘选它绝对没错!
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被称为贵妇洗面奶中性价比高的洁面产品,质地是柔润的膏体起泡后细腻柔滑而且丰富,有效的温和洁净皮肤,含有滋润配方L-丝氨酸等珍贵成分,带给肌肤水润保湿护理。
变速齿轮通过修改API函数GETTICKCOUNT和TIMEGETTIME骗过了游戏和程序的定时器导致游戏和程序速度看起来被改变。下面以GETTICKCOUNT为例进行分析:原本的GETTICKCOUNT汇编:
kernel32!gettickcount mov gs,[bffcaea18]
mov eax,gs:[00000000]
sub edx,edx
mov gs,dx
ret
变速齿轮修改后的GETTICKCOUNT汇编:
kernel32!gettickcount
这里是关键-->jmp 840500d9(840500d9并不是绝对的)
add [eax],al
add [ecx+00000000],ah
sub edx,edx
mov gs,dx
ret
可以看出变速齿轮修改了gettickcount的代码,当游戏和程序使用gettickcount时就会自动跳转到840500d9处执行。再看看840500d9处的代码汇编:
840500d9:CLI
push ebp
mov ebp,esp
push ebx
push ecx
push edx
push esi
push edi
call 840500e7
840500e7:pop edi
xor di,di
mov esi,edi
add esi,00402051
sub esi,00401f0b
push esi
call edi
call 84050101
84050101:pop edi
xor di,di
call [edi+0000fef0]
call 84050110
84050110:sub eax,[edi+0000ff30]
mul dword,ptr[edi+0000ff30]
mov ebx,00100000
div ebx
add eax,[edi+0000fe20]
push eax
mov eax,00402072
sub eax,00401f08
add eax,edi
push eax
call edi
pop eax
pop edi
pop esi
pop edx
pop ecx
pop ebx
pop ebp
sil
ret
以上正是变速齿轮变速的核心所在。(GETTICKCOUNT返回的是EAX的值你可以对EAX进行跟踪)
下面说一下变速齿轮挂接API的方法:首先变速齿轮在MMF区(WIN9X/ME)申请一块内存,把上面的代码从程序中移到该内存。使用修改描述符的方法从应用程序级跳到核心级(具体可查看《电脑编程技巧与维护》2000年第6期34页)修改GETTICKCOUNT开头的代码使之指向申请的内存的首地址实现挂接。
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